Compact Cryo Two-Photon Microscope

High submicron resolution imaging during freezing and heating based on near infrared femtosecond laser technology

-196°C - +600°C (77K – 873K)

cooling rates: 0.01K/min - 150K/min

• Label-free autofluorescence of frozen samples
• Fluorescence Lifetime Imaging (FLIM)
• Second Harmonic Generation (SHG)
• Microspectroscopy

Cryopreservation, thermostress, climate change,
optimization of cryogenic protocols,
High Tech tool for cryobanks,
Human, animal, plant tissue/ cells/ minerals


Two photon cryo FLIM microscopy of plant leaves (Arabidopsis thaliana). Chlorophyll fluorescence in intratissue chloroplasts. High spatial and temporal resolution (300 nm / 200 ps).

Technical data

• compact turn-key tunable Ti:Sapphire femtosecond laser
laser pulse width: 100 fs - 200 fs
repetition frequency: 80 MHz
laser power: < 1.3 W
wavelength range. 710-920 nm
• full-frame scanning, region-of-interest (ROI) scanning, line scanning, single-point illumination (spot scan)
• typical FOV: 250 μm x 250 μm (horizontal); WD: < 2 mm
• typical spatial resolution: < 0.5 μm (horizontal); < 2 μm (vertical)
• typical temporal resolution: 200 ps (TCSPC, up to 256 time channels)
• focusing optics: 40x NA 1.3 (standard), other objectives possible
• control and image processing software (JenLab Control, JenLab Image)
• temperature range -196°C (nitrogen) - +600°C (77K - 873K)
• cooling rates: 0.01K/min - 150K/min
• power requirements: 230 VAC (50 Hz) or 115 VAC (60 Hz)
• CE certified
• 700x520x800mm³ (without laser)

Notes: These specifications are subject to change without notice.

Breunig, Tümer, König. Multiphoton imaging of freezing and heating effects in plant leaves.
J Biophotonics (2012), in press

Summary of the 2PM™-Cryo in Adobe PDF format (click here, 1,4 MB)

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